Bio-larvicidal Potential of Betel Leaves (Piper betle L) Ethanolic Extract in Addition of PEG 400 Diluent on Aedes aegypti Larvae

Dengue hemorrhagic fever (DHF) is a kind of vector transmitted disease, by Aedes aegypti. It one major public health problem around the world, including Indonesia, because it may lead to epidemics and death in short time. The use plant extracts as bio-larvicidal thought be promising solution, them betel leaves (Piper betle L). addition polyethylene glycol (PEG) diluent increase dispersity extract water which larval medium growth. Objectives: To determine potential 95% ethanolic (EEBL) PEG 400 on aegypti mortality. Material Method: Betel were extracted maceration using ethanol. There are two kinds EEBL concentration used, 0.2% dan 0.4%. was also added diluent. samples this study larvae at instar III-IV, with total larvae. Evaluation performed 6, 12, 18, 24 hours. data obtained then analyzed Kruskall Wallis test post-hoc Mann Whitney test. Result: In all treatment groups, mortality reached 100% From test, p-value <0.05. comparation between groups positive control group >0.05, negative Conclusion: known that 0.4% effective temephos larvicides